Chemiluminescent Assay for Phosphatidylcholine Hydroperoxide.
نویسندگان
چکیده
منابع مشابه
Chemiluminescent simultaneous determination of phosphatidylcholine hydroperoxide and phosphatidylethanolamine hydroperoxide in the liver and brain of the rat.
The quantification of phospholipid hydroperoxides in biological tissues is important in order to know the degree of peroxidative damage of membrane lipids. For this purpose, optimal conditions for the chemiluminescent simultaneous assay of phosphatidylcholine hydroperoxide (PCOOH) and phosphatidylethanolamine hydroperoxide (PEOOH) in rat liver and brain were determined. A chemiluminescence dete...
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To compare the peroxidative susceptibilities of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) in liposomes, multilamellar vesicles (MLVs) were prepared with equimolar L-alpha-dilinoleoyl PC (DLPC) and L-alpha-dilinoleoyl PE (DLPE), and with soya PC and soya PE having a uniform constituent fatty acids. The hydroperoxide formation at 37 degrees C in the presence of a water-soluble ra...
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Plasma glutathione peroxidase (GSHPx) has been suggested to reduce submicromolar levels of free fatty acid hydroperoxides and phosphatidylcholine hydroperoxides (PC-OOH), and therefore these hydroperoxides are undetectable in human blood plasma. The capacity for the reduction should be about 2.5 microM as the level of glutathione in human plasma is about 5 microM. However, 2 h of aerobic incuba...
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BACKGROUND The basal lipid peroxide concentration in the plasma of patients with hyperlipidemia may be related to atherosclerosis. Quantitative determination of lipid peroxides in the plasma is an important step in the overall evaluation of the biochemical processes leading to oxidative injury. Unfortunately, the currently available methods for lipid peroxidation lack specificity and sensitivit...
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Quantitative analysis of plasma phosphatidylcholine hydroperoxide (PCOOH) is an important step in evaluating the biochemical processes leading to oxidative injury. However, secondary products of lipid peroxidation are now used as indices. One hundred nine alcoholic patients, aged 22-81 years (mean +/- SEM, 52.0 +/- 1.3 years), and 21 healthy volunteers, aged 41-79 years (51.2 +/- 2.2 years), pa...
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ژورنال
عنوان ژورنال: Analytical Sciences
سال: 1997
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.13.311